Thermo Fisher Scientific Cystatin S Polyclonal Antibody
Human Cystatin S 단백질을 인식하는 Goat Polyclonal Antibody로, Western blot과 Immunoprecipitation에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 보존제 무첨가. 인간 시료에 반응하며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunoprecipitation (IP) | 25 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Cystatin S (Ser21–Ala141) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609825 |
Product Specific Information
- In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) Cystatin SA and rhCystatin SN is observed.
- 5% cross-reactivity with rhCystatin C and rhCystatin D.
- Less than 1% cross-reactivity with rhCystatin A, rhCystatin B, rhCystatin E/M, rhFetuin A, and rhFetuin B.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level: <0.10 EU per 1 µg of antibody (LAL method).
Target Information
The cystatin superfamily includes proteins containing multiple cystatin-like sequences. Some act as active cysteine protease inhibitors, while others have lost or never acquired this activity.
There are three inhibitory families:
- Type 1 cystatins (stefins)
- Type 2 cystatins
- Kininogens
Type 2 cystatins are cysteine proteinase inhibitors found in various human fluids and secretions. The cystatin locus on chromosome 20 contains most of the type 2 cystatin genes and pseudogenes.
This gene encodes a type 2 salivary cysteine peptidase inhibitor, an S-type cystatin, highly expressed in saliva, tears, and seminal plasma. It exhibits antibacterial and antiviral activity, suggesting a protective role.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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