
Thermo Fisher Scientific ID2 Monoclonal Antibody (4E12G5)
Human ID2 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, Flow Cytometry, ELISA에 사용 가능. 고순도 Protein G 정제, 1 mg/mL 농도, PBS + 0.05% sodium azide 용액 형태. 연구용으로 ID2 발현 및 기능 연구에 적합.
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Thermo Fisher Scientific ID2 Monoclonal Antibody (4E12G5)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4E12G5 |
| Immunogen | Purified recombinant fragment of human ID2 (amino acids 1–134) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at −20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538566 |
Product Specific Information
MA5-17095 targets ID2 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human ID2 (amino acids 1–134) expressed in E. coli.
MA5-17095 detects ID2, which has a predicted molecular weight of approximately 15 kDa.
Target Information
The inhibitor of DNA binding 2 (ID2) protein is essential for promoting the development of various cell types including natural killer cells, erythroid precursors, and CD8+/CD4+ T-cells.
A lack of ID2 promotes B-cell development. The protein associates with ubiquitously expressed E proteins and other basic helix-loop-helix (HLH) family members, preventing them from binding DNA and activating transcription.
Activation of ID2 is critical for the development of the natural killer cell lineage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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