Thermo Fisher Scientific SP1 Recombinant Rabbit Monoclonal Antibody (ARC0128)
SP1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, ELISA, ChIP 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 정제, 1.5 mg/mL 농도, -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| ELISA | 1 µg/mL |
| ChIP assay (ChIP) | 5 µg antibody for 10–15 µg chromatin |
| ChIP-sequencing (ChIP-Seq) | 1:50–1:100 |
| CUT&RUN (C&R) | 10⁵ cells / 1 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0128 |
| Immunogen | Synthetic peptide within amino acids 500–600 of human SP1 (P08047) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849233 |
Product Specific Information
Immunogen sequence:
TTLTPIASAA SIPAGTVTVN AAQLSSMPGL QTINLSALGT SGIQVHPIQG LPLAIANAPG DHGAQLGLHG AGGDGIHDDT AGGEEGENSP DAQPQAGRRT R
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in cell growth, apoptosis, differentiation, and immune responses. SP1 activity is highly regulated by post-translational modifications such as phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation. It also binds the PDGFR-alpha G-box promoter and may modulate cellular response to DNA damage. SP1 is implicated in chromatin remodeling and plays a role in recruitment of SMARCA4/BRG1 on the c-FOS promoter, as well as in regulation of FE65 gene expression.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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