Thermo Fisher Scientific ERK2 Recombinant Rabbit Monoclonal Antibody (2K9N9)
ERK2 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ELISA에 사용 가능. 높은 특이성과 재현성을 제공하며, 인간, 마우스, 랫트 시료에 반응. 연구용으로 MAPK 신호전달 경로 분석에 적합.
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Thermo Fisher Scientific ERK2 Recombinant Rabbit Monoclonal Antibody (2K9N9)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2K9N9 |
| Immunogen | Synthetic peptide corresponding to amino acids 200–300 of human ERK2 (ERK1/2, P27361/P28482) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2849229 |
Product Specific Information
Immunogen sequence:
FLTEYVATRW YRAPEIMLNS KGYTKSIDIW SVGCILAEML SNRPIFPGKH YLDQLNHILG ILGSPSQEDL NCIINMKARN YLQSLPSKTK VAWAKLFPKS D/LNSKGYTK SIDIWSVGCI LAEMLSNRPI FPGKHYLDQL NHILGILGSP SQEDLNCIIN LKARNYLLSL PHKNKVPWNR LFPNADSKAL DLLDKMLTFN PHK
Target Information
MAPK1 (ERK2) is a serine/threonine kinase acting as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases (ERKs) serve as integration points for multiple biochemical signals and regulate various cellular processes including proliferation, differentiation, transcription regulation, and development. Activation occurs via phosphorylation by upstream kinases, leading to nuclear translocation and phosphorylation of nuclear targets.
Notes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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