Thermo Fisher Scientific GATA3 Recombinant Rabbit Monoclonal Antibody (ARC0115)
GATA3 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단일클론 항체로, WB, IHC, ELISA, IP, ChIP, ChIP-Seq 등 다양한 실험에 적합합니다. 높은 특이성과 재현성을 제공하며, HEK293 세포에서 발현된 IgG 형 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
| ChIP assay (ChIP) | 5 µg antibody for 10–15 µg Chromatin |
| ChIP-sequencing (ChIP-Seq) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0115 |
| Immunogen | Recombinant fusion protein containing sequence corresponding to amino acids 1–200 of human GATA3 (P23771) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849230 |
Product Specific Information
Immunogen sequence:
MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LSHSYMDAAQ YPLPEEVDVL FNIDGQGNHV PPYYGNSVRA TVQRYPPTHH GSQVCRPPLL HGSLPWLDGG KALGSHHTAS PWNLSPFSKT SIHHGSPGPL SVYPPASSSS LSGGHASPHL FTFPPTPPKD VSPDPSLSTP GSAGSARQDE KECLKYQVPL PDSMKLESSH
Target Information
The genes for all four subunits of the T-cell antigen receptor (alpha, beta, gamma, and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all four TCR genes.
GATA3 is an enhancer-binding protein containing a zinc finger domain and is abundantly expressed in the T-lymphocyte lineage. It participates in T-cell receptor gene activation through enhancer binding. Labastie et al. (1994) cloned the human gene, which comprises six exons distributed over 17 kb of DNA. Its two zinc fingers are encoded by separate exons highly conserved with those of GATA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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