Thermo Fisher Scientific ARHGEF1 Polyclonal Antibody
ARHGEF1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot, ICC/IF, IP에 사용 가능. 사람, 생쥐, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 206–251 of human ARHGEF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553879 |
Product Specific Information
- Detects endogenous ARHGEF1 protein at a molecular weight of approximately 115 kDa.
- Purity >95% by SDS-PAGE.
Target Information
The Ras superfamily of GTPases, including Ras, Rho/Rac, Sar, Rab, ARF, and Ran subfamilies, regulates various cellular processes such as cytoskeletal rearrangement, nuclear signaling, and cell growth.
These GTPases act as molecular switches that alternate between active (GTP-bound) and inactive (GDP-bound) states, with activation facilitated by guanine nucleotide exchange factors (GEFs).
Dbl-related proteins represent a family of GEFs that catalyze exchange activity for Ras family members, including FGD1, Lsc, RhoGEF p115, Lfc, Lbc, and Brx.
Among these, RhoGEF p115 specifically activates Rho GTPases but not Rac, Cdc42, or Ras.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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