Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (5A8E5), Biotin, THE

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

-

ELISA (ELISA)

0.05-0.2 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

5A8E5

Immunogen

A synthetic peptide (DYKDDDDK) coupled to KLH

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• FITC
• HRP
• iFluor 488
• iFluor 555
• iFluor 647
• Request custom conjugation

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with BSA

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.

GenScript THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse is the biotin conjugated THE™ DYKDDDDK Tag Antibody, mAb, Mouse (A00187) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.