Thermo Fisher Scientific IkB beta Polyclonal Antibody
인간 NFKBIB/IkB-beta 단백질을 인식하는 Rabbit Polyclonal 항체입니다. ELISA 및 IP에 사용 가능하며, 단백질 A 및 항원 친화 크로마토그래피로 정제되었습니다. 방부제 무첨가 제품으로 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution / Amount |
|---|---|
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 0.5–2 µL/mg of lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human NFKBIB/IkB-beta Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2788121 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human NFKBIB/IkB-beta.
Target Information
The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with inhibitory IkB proteins. Most agents that activate NF-kB do so through a common pathway involving phosphorylation-induced, proteasome-mediated degradation of IkB.
The key regulatory step involves activation of a high molecular weight IkB kinase (IKK) complex, composed of IKK alpha and IKK beta (catalytic subunits) and IKK gamma (regulatory subunit). Activation occurs via phosphorylation at Ser177 and Ser181 in IKK beta (Ser176 and Ser180 in IKK alpha), leading to conformational changes and kinase activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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