Thermo Fisher Scientific DFFB Monoclonal Antibody (4B8)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

ELISA

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

A suggested positive control for this product is A431 cells.

Target Information

Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Apoptosis, a cell death process removing toxic and/or useless cells during mammalian development, can be induced by various stimuli such as death factors of the tumor necrosis factor family, UV irradiation, anticancer drugs, and survival factor deprivation.
During apoptosis, cells and nuclei shrink and fragment, and chromosomal DNA degrades into nucleosomal units. CAD, activated by caspases, forms a complex with its inhibitor ICAD. ICAD assists proper folding of CAD and inhibits its DNase activity. When caspases are activated, ICAD is cleaved, releasing CAD to enter the nucleus and cleave chromosomal DNA.
Apoptosis regulation involves internal and external signals controlled by the balance between pro- and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs). Fourteen members of the caspase family have been identified, seven of which mediate apoptosis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.