Thermo Fisher Scientific Syk Monoclonal Antibody (8H5)
Syk 단백질을 인식하는 Mouse IgG1 단일클론 항체로, ELISA 및 면역침강(IP)에 사용 가능. THP-1 세포를 양성 대조군으로 권장. SYK 단백질 연구 및 신호전달 경로 분석에 적합. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 2 µL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8H5 |
| Immunogen | Synthetic peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2538674 |
Product Specific Information
A suggested positive control for this product is THP-1 cells.
Target Information
Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential drug targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer.
Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes.
Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motifs of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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