Thermo Fisher Scientific PLS3 Polyclonal Antibody
PLS3 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, ELISA, IP에 적합합니다. Human, Mouse, Rat 시료에 반응하며, PBS/glycerol buffer에 보관됩니다. Apoptosis 관련 연구 및 PKC-delta 인산화 연구에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 1–240 of human PLS3 (NP_0050232) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.08 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806359 |
Product Specific Information
Immunogen sequence:
MDEMATTQIS KDELDELKEA FAKVDLNSNG FICDYELHEL FKEANMPLPG YKVREIIQKL MLDGDRNKDG KISFDEFVYI FQEVKSSDIA KTFRKAINRK EGICALGGTS ELSSEGTQHS YSEEEKYAFV NWINKALEND PDCRHVIPMN PNTDDLFKAV GDGIVLCKMI NLSVPDTIDE RAINKKKLTP FIIQENLNLA LNSASAIGCH VVNIGAEDLR AGKPHLVLGL LWQIIKIGLF
Positive Samples: HeLa, MCF-7, BT-474, A-431, HepG2
Cellular Location: Cytoplasm
Target Information
Phospholipid scramblases (PLS) are enzymes that translocate phospholipids bidirectionally between the inner and outer leaflets of the plasma membrane and are involved in apoptosis.
PLS1 and PLS3 are substrates of Protein kinase C (PKC) delta and become phosphorylated during apoptosis. PKC-delta translocates to mitochondria during apoptosis and phosphorylates PLS3.
Overexpression of PLS3 in HEK293 cells enhances apoptosis induced by UV-irradiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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