Thermo Fisher Scientific TGM2 Monoclonal Antibody (OTI1C4), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:500

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of ~1 mg/mL.
• For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Transglutaminase (TGase) catalyzes protein crosslinking by forming ε-(γ-glutamyl) lysine isopeptide bonds and requires Ca²⁺ binding for activity. In mammals, eight distinct TGase isoenzymes exist. Tissue transglutaminase (tTGase or TGase 2) contains four domains: N-terminal β-sandwich, catalytic core, and two C-terminal β-barrel domains.
tTGase is implicated in cell death, proliferation, differentiation, and receptor-mediated endocytosis. In Alzheimer’s disease, elevated tTGase activity leads to polymerization of proteins such as Aβ peptide, β-amyloid precursor protein, and tau, contributing to neurofibrillary tangle formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.