Thermo Fisher Scientific MERS Coronavirus Spike Protein S1 Monoclonal Antibody (3873)

Thermo Fisher Scientific MERS Coronavirus Spike Protein S1 Monoclonal Antibody (Clone 3873)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Specific for the S1 domain of the spike protein of the MERS virus.
• Does not cross-react with the SARS Coronavirus.
• Purified preparations consist of >90% pure mouse monoclonal antibody purified from ascites fluid by Protein A chromatography.
• Formulated at 100 µg/mL in 0.01 M phosphate-buffered saline (pH 7.2) with 0.1% sodium azide.
• Each vial contains 1.0 mL.
• No stabilizing proteins included.
• Store at 4°C until use.
• Working dilution should be determined by the user. Suggested ranges: ICC/IF 1:10–1:50, ELISA 1:20–1:200.

Target Information

The spike (S) glycoprotein of coronaviruses contains protrusions that bind to specific host cell receptors, playing a crucial role in host specificity and viral infectivity. The term peplomer refers to a grouping of heterologous proteins on the virus surface that function together.

The spike (S) glycoprotein is essential for virus binding and entry into host cells. In SARS-CoV, the S protein mediates membrane fusion and cell entry, acting as a major immunogen and target for entry inhibitors.
The S protein consists of two subunits:

• S1 subunit: Contains a receptor-binding domain that engages with the host cell receptor ACE2.
• S2 subunit: Mediates fusion between viral and host cell membranes.

The S protein contributes to neutralizing antibody and T-cell responses, as well as protective immunity during infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.