Thermo Fisher Scientific MERS Coronavirus Spike Protein S1 Monoclonal Antibody (3873)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:50

-

ELISA (ELISA)

1:20-1:200

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3873

Immunogen

Proprietary sequence

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2942510

Product Specific Information

Specificity: Specific for the S1 domain of the spike protein of the MERS virus. Does not cross react with the SARS Coronavirus.

Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography. The final preparation is formulated to a protein concentration of 100 µg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide. Each vial contains 1.0 ml. This product contains no stabilizing proteins and should be stored at 4C until ready for use. Working dilution must be determined by the user. Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200. for ELISA.

Target Information

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term peplomer is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.