Thermo Fisher Scientific IkB beta Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Positive Control: Target recombinant protein
• Predicted Reactivity: Mouse (84%), Rhesus Monkey (97%), Bovine (83%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins. Most agents that activate NF-kB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB.
The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex whose catalysis is carried out by three tightly associated IKK subunits: IKK alpha and IKK beta (catalytic subunits) and IKK gamma (regulatory subunit).
Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKK beta (Ser176 and Ser180 in IKK alpha), which causes conformational changes, resulting in kinase activation.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.