Thermo Fisher Scientific Phospho-Tau (Ser519) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Tau only when phosphorylated at Ser519, a site historically referenced as Ser202.

Target Information

Tau is a neuronal microtubule-associated protein predominantly localized on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules, while the N-terminus associates with neural plasma membrane components, functioning as a linker between the two. Axonal polarity is determined by TAU/MAPT localization within the neuronal cell body domain defined by the centrosome.

Short isoforms contribute to cytoskeletal plasticity, whereas longer isoforms aid in stabilization. In its hyper-phosphorylated form, Tau constitutes paired helical filaments (PHF), the main component of neurofibrillary tangles in Alzheimer’s disease (AD). Hyper-phosphorylation disrupts Tau’s microtubule-binding ability, leading to microtubule destabilization and neuronal degeneration in AD brains.

Tau phosphorylation is mediated by several kinases, including GSK-3β, protein kinase A (PKA), cyclin-dependent kinase 5 (CDK5), and casein kinase II. Hyper-phosphorylated Tau is also observed in other neurodegenerative disorders such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.