Thermo Fisher Scientific Phospho-Tau (Ser733) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Tau only when phosphorylated at Ser733, which site has been historically referenced as Ser416.

Target Information

Tau is a neuronal microtubule-associated protein predominantly found on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules, while the N-terminus binds neural plasma membrane components, suggesting that Tau functions as a linker protein between both structures.

Axonal polarity is determined by TAU/MAPT localization within the neuronal cell body domain defined by the centrosome. Short isoforms contribute to cytoskeletal plasticity, whereas longer isoforms are involved in stabilization.

In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), which constitute neurofibrillary lesions in Alzheimer’s disease (AD) brain tissue. Hyper-phosphorylation impairs Tau’s microtubule-binding function, leading to microtubule destabilization and neuronal degeneration in AD.

Numerous serine/threonine kinases phosphorylate Tau, including GSK-3β, PKA, CDK5, and casein kinase II. Hyper-phosphorylated Tau is also found in neurofibrillary lesions associated with other central nervous system disorders such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.