Thermo Fisher Scientific RAD54 Polyclonal Antibody
인간 RAD54 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot, ELISA, IP에 사용 가능. 친화 크로마토그래피로 정제된 액상 항체이며, 1 mg/mL 농도. -20°C 보관, 반복 동결·해동은 피해야 함. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | — |
| ELISA | Assay-dependent | — |
| Immunoprecipitation (IP) | Assay-dependent | — |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near aa 1–25 of Human RAD54 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
BLAST analysis indicates 100% homology of the immunizing sequence with human RAD54. Cross-reactivity with RAD54 homologues from other species may not occur due to sequence variation.
Target Information
The protein encoded by this gene belongs to the DEAD-like helicase superfamily and shares similarity with Saccharomyces cerevisiae Rad54. It is involved in homologous recombination and repair of DNA double-strand breaks. Binding of this protein to double-strand DNA induces topological changes that facilitate homologous DNA pairing and stimulate recombination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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