Thermo Fisher Scientific Perilipin 1 Polyclonal Antibody
Perilipin 1 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 사용 가능. Rat 및 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. -20°C에서 보관하며 연구용으로 적합.
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- PA11051
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:4,000 | View 8 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | – | View 1 publication |
| Immunohistochemistry (Frozen) (IHC-F) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues C E(502) P I L G R T Q Y S Q L R K K S(517) of rat Perilipin A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2167579 |
Product Specific Information
PA1-1051 detects perilipin A from rat and 3T3-L1 cell extracts.
It has been successfully used in Western blot procedures, detecting an approximately 62 kDa protein representing perilipin A from 3T3-L1 cell extract.
The immunogen is a synthetic peptide corresponding to residues C E(502) P I L G R T Q Y S Q L R K K S(517) of rat Perilipin A, with 93% sequence conservation in humans.
The immunizing peptide (Cat. # PEP-169) is available for use in neutralization and control experiments.
Target Information
Adipose tissue functions as an energy reserve and is tightly regulated. Adipocytes secrete various proteins including lipoprotein lipase (LPL), leptin, Acrp30, resistin, and perilipin.
Perilipin is an intracellular neutral lipid droplet protein regulated hormonally and localized to the surface of lipid droplets.
Upon lipolytic stimulation, perilipin is phosphorylated by protein kinase A and modulates hormone-sensitive lipase (HSL) activity, affecting triacylglycerol hydrolysis.
Perilipin expression is restricted to adipocytes and steroidogenic cells, with two isoforms (A and B) produced by alternative splicing of a single gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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