Thermo Fisher Scientific Perilipin 1 Polyclonal Antibody, Alexa Fluor 488
Alexa Fluor 488으로 표지된 Perilipin 1 폴리클로날 항체로, 지방세포 내 중성지질 방울 단백질 검출에 적합. IHC, ICC/IF 등 다양한 형식에서 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 안정성 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Perilipin 1 Polyclonal Antibody, Alexa Fluor 488
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues C E(502) P I L G R T Q Y S Q L R K K S(517) of rat Perilipin A |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1.0 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723453 |
Product Specific Information
PA1-1051 detects perilipin A from rat and 3T3-L1 cell extract.
The PA1-1051 immunogen is a synthetic peptide corresponding to residues C E(502) P I L G R T Q Y S Q L R K K S(517) of rat Perilipin A.
This sequence is 93% conserved in humans.
The PA1-1051 immunizing peptide (Cat. # PEP-169) is available for use in neutralization and control experiments.
Target Information
Adipose tissue acts as an energy reserve and is strictly regulated. Adipocytes produce and secrete key proteins such as lipoprotein lipase (LPL), leptin, Acrp30, resistin, and perilipin.
Perilipin is an intracellular neutral lipid droplet protein localized to the surface of lipid droplets and hormonally regulated.
Upon lipolytic stimulation, perilipin is phosphorylated by protein kinase A and inhibits hormone-sensitive lipase (HSL), which mediates triacylglycerol hydrolysis.
Perilipin expression is limited to adipocytes and steroidogenic cells. Two isoforms, Perilipin A and B, are known and arise from differential splicing of a single gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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