Thermo Fisher Scientific CD161 Monoclonal Antibody (HP-3G10), PE, eBioscience

Thermo Fisher Scientific CD161 Monoclonal Antibody (HP-3G10), PE, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 5 publications

Product Specifications

Additional Formats Available:

• Alexa Fluor 488
• APC
• eFluor 450
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Custom conjugation available

Product Specific Information

This HP-3G10 monoclonal antibody reacts with CD161 (NKR-P1A), a member of the C-type lectin superfamily. CD161 is expressed on natural killer cells and various T cell subsets, including memory/effector CD4+ T cells, CD8+ T cells, gamma delta TCR T cells, and a subset of CD3+ thymocytes. It is also found on human Th17 CD4+ T cells. The receptor’s function is unclear but may have a stimulatory role. Lectin-like transcript-1 (LLT1), also known as osteoclast inhibitory lectin, is identified as its ligand.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on CD4+ human peripheral T cells under Th17 polarizing conditions. Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

KLRB1 (CD161) is a type II membrane protein expressed by NK cells and a subset of CD3⁺ T cells. It forms a homodimeric cell surface protein (40–44 kDa) and plays an inhibitory role on NK cells. Activation of KLRB1 stimulates acid sphingomyelinase/SMPD1 and increases intracellular ceramide, as well as AKT1/PKB and RPS6KA1/RSK1 kinase activation. KLRB1 binds to carbohydrate epitopes such as Gal-α(1,3)Gal and N-acetyllactosamine, and interacts with CLEC2D/LLT1 to inhibit NK cell-mediated cytotoxicity and interferon-γ secretion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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