Invitrogen™

PureLink™ Pro 96 total RNA Purification Kit

The PureLink™ Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of자세히 알아보기

The PureLink™ Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples (Figures 1 and 2).
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design
The PureLink™ Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

사양

Isolation TechnologySilica Plate

정제 시간25 min. (After sample homogenization)

용리량45–170 μL

샘플 종류Bacteria, Cells, Plant, Tissue, Yeast

최종 제품 유형Total RNA

고처리량 호환성High-throughput Compatible

용도(장비)Biomek FX, Robotic Liquid Handling Systems, Eppendorf epMotion TECAN Freedom Evo, TECAN Genesis

용도(애플리케이션)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling

수량4 Plates

스케일Mini

배송 조건Room Temperature

시작 물질 양Bacteria: ≤109 cells
Cells: varies
Plant: ≤150 mg
Tissue: ≤100 mg
Yeast: ≤1.8 mL

수율Up to 33 μg

Unit SizeEach