Thermo Fisher Scientific TRAF-1 Monoclonal Antibody (1F3), eBioscience
인간 TRAF-1 단백질을 인식하는 단클론 항체로, 세포 생존 및 신호전달 연구에 적합합니다. Western blot, IHC, ICC/IF에 사용 가능하며, 고순도(>90%)로 제조되었습니다. 4°C 보관, PBS 버퍼 내 0.09% sodium azide 포함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-Dependent |
| Immunohistochemistry (Paraffin) (IHC-P) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573037 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
- eFluor 570 (Cat. No. 41-9791-82)
- Request custom conjugation
Product Specific Information
The monoclonal antibody 1F3 recognizes human TRAF-1, a multifunctional adapter molecule in the TNF receptor associated factor (TRAF) family. TRAF-1 regulates cell survival and apoptosis, mediates NF-κB and JNK signaling, and forms a heterodimeric complex with TRAF-2. It interacts with receptors such as CD30, CD40, CD357 (AITR), and cytoplasmic proteins including NIK, RIP2, A20, and TRIP. Expression of TRAF-1 can be induced by Epstein-Barr Virus (EBV) and TNF-α.
Applications Reported: Suitable for western blotting, microscopy, immunohistochemistry, and immunocytochemistry.
Applications Tested: Validated for IHC of formalin-fixed paraffin-embedded human tissue using low pH antigen retrieval at ≤5 µg/mL. Optimal dilution should be determined experimentally.
Target Information
The TRAF-1 protein is a member of the TNF receptor (TNFR) associated factor family, mediating signal transduction from TNFR superfamily receptors. TRAF-1 and TRAF-2 form a complex essential for TNF-α-mediated activation of MAPK8/JNK and NF-κB pathways. This complex also interacts with inhibitor-of-apoptosis proteins (IAPs), contributing to anti-apoptotic signaling. Expression can be induced by Epstein-Barr Virus (EBV), and the viral LMP1 protein interacts with TRAF proteins, linking EBV-mediated B cell transformation to TNFR signaling. Multiple transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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