Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Biotin, eBioscience

Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Biotin, eBioscience™

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 615
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation available

Product Specific Information

Description:
The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed by developing and mature B cells, but not plasma cells. Low-level expression is also found on a subset of mature T cells. CD20 is implicated in B-cell activation.

Applications Reported:
Suitable for flow cytometric analysis and immunohistology staining of frozen tissue sections.

Applications Tested:
Validated by flow cytometric analysis of normal human peripheral blood cells. Recommended use ≤0.25 µg per test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and malignant B cells, as well as a subset of T lymphocytes and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20’s association with lipid rafts and BCR aggregation affects its surface behavior. It remains membrane-bound after antibody binding, making it an effective therapeutic target for B-cell depletion.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.