Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Biotin, eBioscience
인간 CD20을 인식하는 2H7 클론 단일클론 항체로, 비오틴 결합 형태입니다. Flow cytometry 및 frozen tissue IHC에 적합하며, B세포 활성화 연구에 활용됩니다. 고순도 Affinity chromatography 정제, 4°C 보관 권장.
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Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Biotin, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 0.25 µg/test | View 15 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H7 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657690 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV 395
- Brilliant UV 496
- Brilliant UV 615
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 506
- FITC
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Yellow 590
- NovaFluor Yellow 660
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation available
Product Specific Information
Description:
The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed by developing and mature B cells, but not plasma cells. Low-level expression is also found on a subset of mature T cells. CD20 is implicated in B-cell activation.
Applications Reported:
Suitable for flow cytometric analysis and immunohistology staining of frozen tissue sections.
Applications Tested:
Validated by flow cytometric analysis of normal human peripheral blood cells. Recommended use ≤0.25 µg per test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and malignant B cells, as well as a subset of T lymphocytes and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20’s association with lipid rafts and BCR aggregation affects its surface behavior. It remains membrane-bound after antibody binding, making it an effective therapeutic target for B-cell depletion.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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