Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), Biotin, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Brilliant UV 395 / 496 / 615 / 737 / 805
• Brilliant Violet 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 610-30S
• NovaFluor Red 685 / 710
• NovaFluor Yellow 570 / 590 / 660
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5 / PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The M1/69 monoclonal antibody reacts with the mouse CD24 molecule (Heat Stable Antigen, HSA).
This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage.
CD24 is a variably glycosylated molecule showing heterogeneity in molecular mass across different cell lineages.
The expression of CD24 is used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes, optimal at ≤ 0.125 µg/test (100 µL final volume).
Cell number should be determined empirically (10⁵–10⁸ cells/test).
Careful titration is recommended for optimal performance.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD24 is a two-chain GPI-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B to mature B-cells.
Expression is low or absent in plasma cells.
CD24 is also expressed on most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas.
It may play a role in the regulation of B-cell proliferation, maturation, and autoimmunity control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.