Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), Biotin, eBioscience

Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Brilliant UV 805
• eFluor 450
• eFluor 660
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein expressed on resting conventional B cells and modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble forms of the antigen are biologically active. CD23 serves as a low affinity receptor for IgE and plays a role in IgE response regulation and B-cell activation. CD21 is known to bind CD23.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg per test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.

Target Information

CD23 is a 45 kDa glycoprotein present on a subset of peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. It is identical to the low-affinity IgE receptor on B cells. Expression is observed in neoplastic cells of B cell chronic lymphocytic leukemia and certain lymphomas. Functionally, CD23 is involved in B cell growth, differentiation, and IgE production. Soluble CD23 acts as a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.