Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

본 항체는 다양한 형광 표지 형식으로 제공됩니다.

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Brilliant UV / Violet 시리즈
• eFluor 450, 506, 660
• FITC, PE, PE-Cyanine 시리즈
• PerCP-Cyanine5.5
• Super Bright 시리즈
• NovaFluor 시리즈
• Functional Grade 등
  Request custom conjugation

Product Specific Information

Description:
The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together with CD21, CD81, and MHC class II, CD19 forms a multimolecular complex associated with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells.

Applications Reported:
This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.25 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and may also be found on myeloid leukemia cells. CD19 serves as a key regulator of B cell activation and proliferation, acting as a co-receptor with the BCR to modulate antigen sensitivity. Mutations in CD19 can result in hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.