Thermo Fisher Scientific IRF7 Monoclonal Antibody (RDP4ND4), PE-Cyanine7, eBioscience

Thermo Fisher Scientific IRF7 Monoclonal Antibody (RDP4ND4), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: -

Product Specifications

Additional Formats

• Alexa Fluor 647
• PE
• Request custom conjugation

Product Specific Information

The monoclonal antibody RDP4ND4 recognizes human interferon regulatory factor 7 (IRF7), a 54 kDa transcription factor essential for antiviral innate immune response and activation of type I IFN genes.
IRF7 is constitutively expressed in plasmacytoid dendritic cells, B cells, and monocytes, and is strongly induced upon viral infection or interferon stimulation.

IRF7 activates IFN-beta and IFN-alpha genes via virus-activated (MyD88-independent) or TLR-activated (MyD88-dependent) pathways. Virus exposure induces IRF7 through TLR7, TLR8, and TLR9, promoting IFN gene expression. Phosphorylation triggers dimerization and nuclear translocation of IRF7, enabling transcriptional activation of target genes. IRF7 expression is upregulated by interferon via the Jak-STAT pathway in a positive feedback loop.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry using stimulated human peripheral blood cells and Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Use at 5 µL (0.125 µg) per test (100 µL final volume). Typical cell range: 10⁵–10⁸ cells/test.

Light Sensitivity: Tandem dye is sensitive to photo-induced oxidation. Protect vial and samples from light.
Fixation: Samples may be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation: 488–561 nm
Emission: 775 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers

Target Information

Interferons (IFNs) are key regulators of antiviral immune mechanisms. IRF7 is a transcription factor involved in activation of virus-inducible genes, including interferon beta chain genes.
It interacts with TLR adaptor proteins MyD88 and Tirp/TRAM, functioning as an intermediate in TLR4 and TLR9 signaling pathways.
At least four spliced isoforms of IRF7 exist, though their functions remain unclear.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.

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