Thermo Fisher Scientific GPNMB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

-

Neutralization (Neu)

0.2-0.8 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Osteoactivin/GPNMB Lys23-Asn502 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GPNMB,uniProtId:Q99P91-1,ncbiNodeId:10090,antigenRange:23-502,antigenLength:574,antigenImageFileName:PA5-47301_GPNMB_Q99P91-1_Goat.svg,antigenImageFileNamePDP:PA5-47301_GPNMB_Q99P91-1_Goat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2605801

Product Specific Information

In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant human (rh) Osteoactivin, rhSyndecan-4, and recombinant mouse Syndecan-4 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.