Thermo Fisher Scientific Human CCL15 (MIP-5) Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Human CCL15 recombinant protein contains 92 amino acids

Molecular weight

10.1 kDa

Class

Recombinant

Type

Protein

Purity

>98% by SDS-PAGE

Endotoxin concentration

<0.1 ng/µg

Activity

ED50 = 1 - 10 ng/mL; determined by the dose-dependent chemotaxis of human T-lymphocytes.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Carrier-Free

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute MIP-5 in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1-1.0% BSA.

Storage: This lyophilized preparation is stable at room temperature for 1 month. The reconstituted preparation should be apportioned into working aliquots and stored at ≤20C° for up to 3 months with a carrier protein. Avoid repeated freeze/thaw cycles.

Target Information

This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.