
Thermo Fisher Scientific Human CCL15 (MIP-5) Recombinant Protein
인간 유래 CCL15 (MIP-5) 재조합 단백질로, T세포 및 단핵구의 화학주성 연구에 적합합니다. E. coli 발현 시스템에서 생산되었으며, >98% 순도를 보장합니다. 기능적 활성 검증 완료, ELISA, WB, IHC 등 다양한 연구용으로 사용 가능합니다.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | Assay-dependent | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Functional Assay | Assay-dependent | |
| Control | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human CCL15 recombinant protein contains 92 amino acids |
| Molecular Weight | 10.1 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 1–10 ng/mL; determined by the dose-dependent chemotaxis of human T-lymphocytes |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution:
Briefly centrifuge vial before opening to collect contents at the bottom.
Reconstitute MIP-5 in sterile, distilled water to a concentration of 0.1–1.0 mg/mL.
Further dilutions should be made in aqueous buffers supplemented with carrier protein (e.g., 0.1–1.0% BSA).Storage:
Lyophilized preparation is stable at room temperature for 1 month.
After reconstitution, aliquot and store at ≤ -20°C for up to 3 months with carrier protein.
Avoid repeated freeze/thaw cycles.
Target Information
This gene is located in a cluster of similar genes on chromosome 17. These genes encode CC cytokines—secreted proteins characterized by two adjacent cysteines. The gene product is chemotactic for T cells and monocytes and acts through the C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into multiple smaller functional peptides. Naturally occurring readthrough transcripts extend into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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