Thermo Fisher Scientific NGF Polyclonal Antibody
NGF 단백질을 표적으로 하는 Sheep 유래 Polyclonal 항체로, Western Blot 및 ELISA에 사용 가능. Lyophilized 형태로 제공되며 Protein G로 정제됨. 다양한 종(인간, 생쥐, 닭 등)에 반응하며 연구용으로 적합.
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- PA118381
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Thermo Fisher Scientific NGF Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–3 µg/mL |
| ELISA | 1–3 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Avian, Chicken, Human, Mouse, Rat |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Native mouse beta NGF purified from submaxillary salivary gland |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1077263 |
Product Specific Information
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
After reconstitution, keep aliquots at -20°C for higher stability, and at 4°C with an appropriate antibacterial agent.
Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for additional stability.
Target Information
Nerve growth factor (NGF) influences the survival and differentiation of various neuronal and nonneuronal cells.
NGF binds to specific cell surface receptors with both high and low affinity sites, but only the high affinity form is capable of signal transduction.
Cross-linking studies with 125I-NGF show that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF, while the low affinity receptor forms an Mr 100,000 complex.
Full-length cDNA clones for human and rat NGF receptor genes reveal >90% homology between species.
NGF regulates growth and differentiation of sympathetic and sensory neurons and stimulates division of embryonic sensory neurons.
Mutations in the NGF gene are associated with hereditary sensory and autonomic neuropathy type 5 (HSAN5), and dysregulation of NGF expression is linked to allergic rhinitis.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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