Thermo Fisher Scientific BAD Monoclonal Antibody (1G5B3)
BAD 단백질을 인식하는 Mouse monoclonal antibody(클론 1G5B3)로 Western blot, Flow cytometry, ELISA에 사용 가능. Human 시료 반응성. Protein G 정제, PBS buffer에 0.05% sodium azide 포함. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1G5B3 |
| Immunogen | Purified recombinant fragment of human BAD (aa 1–168) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898368 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
The protein encoded by BAD is a member of the BCL-2 family, which regulates programmed cell death. BAD promotes apoptosis by forming heterodimers with BCL-xL and BCL-2, counteracting their anti-apoptotic effects. Its proapoptotic activity is regulated by phosphorylation, involving kinases such as AKT and MAP kinase, and phosphatase calcineurin. Alternative splicing produces two transcript variants encoding the same isoform.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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