Takara TB Green Premix Ex Taq II (Tli RNase H Plus)

Overview

• High specificity minimizes primer-dimer formation and nonspecific amplification
• Excels with GC-rich targets
• 2X master mix provides convenience
• High sensitivity: detects fewer than 100 copies
• Compatible with all commonly used qPCR instruments
• Includes TB Green Master Mix for intercalator-based qPCR
• Tli RNase H Plus reduces inhibition from mRNA/cDNA hybrids in qPCR
• Eliminates the need for a separate RNase H digestion step when using low RNase H RT

More Information

Applications

• Gene expression analysis
• qPCR
• Genotyping
• Suppression of primer dimers or off-target products

Components

Two ROX reference dyes are supplied as separate components with Cat. # RR820A, RR820B, RR820L, and RR820W. These reference dyes serve as convenient internal reference standards when normalizing non-PCR generated fluorescence fluctuations; such fluctuations often occur between different wells or over time in different instruments.

Cat. # RR820L and RR820W are larger package sizes (5 ml and 25 ml (5 x 5 ml), respectively) of TB Green Premix Ex Taq II (Tli RNase H Plus). Each of these includes a separate vial of ROX Reference Dye.

Cat. # RR82LR and RR82WR are larger package sizes (5 ml and 25 ml (5 x 5 ml), respectively) with ROX Reference Dye included in the premix.

Product citations

Hong, S. et al. Type II protein arginine methyltransferase 5 (PRMT5) is required for circadian period determination in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 107:21211–21216 (2010).

Ishikawa, M. et al. Downregulation of glutamine synthetase via GLAST suppression induces retinal axonal swelling in a rat ex vivo hydrostatic pressure model. Invest. Ophthalmol. Vis. Sci. 52:6604–6616 (2011).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.