Thermo Fisher Scientific TLR9 Monoclonal Antibody (5G5), FITC
TLR9 단백질을 인식하는 단일클론 항체로, FITC 형광 표지되어 있습니다. 인간 및 생쥐 시료에 반응하며, WB, IHC, Flow Cytometry 등 다양한 면역분석에 사용 가능합니다. 단백질 G로 정제되었으며, 4°C 암조건에서 보관합니다.
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Thermo Fisher Scientific TLR9 Monoclonal Antibody (5G5), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
| Inhibition Assays (IA) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5G5 |
| Immunogen | Purified fusion protein of extracellular domain of human TLR9 (AA 1–815) and human IgG Fc |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_3093972 |
Additional Formats
- Biotin conjugate available (Cat. No. MA5-49116)
- Custom conjugation requests available
Product Specific Information
The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9-transfected HEK293 cells, and is cross-reactive with canine TLR9.
Target Information
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity.
TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns expressed on infectious agents and mediate cytokine production necessary for effective immune responses.
TLR9 is preferentially expressed in immune cell-rich tissues such as spleen, lymph node, bone marrow, and peripheral blood leukocytes. It mediates cellular responses to unmethylated CpG dinucleotides in bacterial DNA, initiating an innate immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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