Thermo Fisher Scientific CCR5 Monoclonal Antibody (45523.111)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

25 µg/mL

-

Flow Cytometry (Flow)

5-10 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

45523.111

Immunogen

Human CCR5 transfected NSO mouse myeloma cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_779631

Product Specific Information

Recommended positive controls: human lymph node.

This antibody does not detect CCR5 on unfixed human PBMCs. The antibody shows no cross reactivity with CCR1, CCR2 or CCR3.

Endotoxin level is less than 0.1 EU as determined by the LAL method.

Target Information

Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.