Thermo Fisher Scientific CCR5 Monoclonal Antibody (45523.111)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive control: human lymph node
• This antibody does not detect CCR5 on unfixed human PBMCs.
• No cross-reactivity with CCR1, CCR2, or CCR3.
• Endotoxin level < 0.1 EU (LAL method).

Target Information

Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Several G protein-coupled receptors, including CCR5, CXCR4, CCR3, CCR2b, and CCR8, have been identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains, facilitating fusion and entry into human white blood cells.

CCR5 is essential for infection by HIV-1, HIV-2, and SIV. Beta-chemokines such as RANTES, MIP-α, and MIP-β act as ligands for CCR5 and can prevent infection by M-tropic HIV-1. CCR5 interacts with the surface CD4-gp120 complex of HIV, leading to membrane fusion and viral entry. The amino-terminal domain and extracellular loops of CCR5 serve as binding sites for HIV. CCR5 mRNA is expressed in lymphoid organs and monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.