Thermo Fisher Scientific Tyrosine Hydroxylase Monoclonal Antibody (185)
Tyrosine Hydroxylase 단백질을 인식하는 mouse monoclonal antibody로, WB, IHC, ICC 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 0.24 mg/mL 농도의 액상 형태로 제공됩니다. 신경전달물질 합성 연구에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:25–1:50 | - |
| Immunohistochemistry (IHC) | - | 8 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:50 | 2 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 185 |
| Immunogen | Recombinant protein corresponding to the C-terminal portion of mouse Tyrosine Hydroxylase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.24 mg/mL |
| Storage Buffer | Tissue culture supernatant |
| Contains | 15 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_795666 |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters such as dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with the cofactor tetrahydrobiopterin (BH4), which binds to the TH catalytic domain to activate the enzyme.
Four human TH mRNA splice variants (hTH1–hTH4) have been identified, sharing identical catalytic domains but differing in their N-terminal regulatory domains. TH catalyzes the conversion of L-tyrosine to L-DOPA and plays a key role in adrenergic neuron physiology.
Abnormal TH activity is associated with neurological and cardiovascular diseases such as Parkinson’s disease, schizophrenia, Segawa syndrome, dystonia, and various cardiovascular disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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