Thermo Fisher Scientific Phospho-PI3K p85/p55 (Tyr458, Tyr199) Recombinant Rabbit Monoclonal Antibody (PI3KY458-1A11), PE
Phospho-PI3K p85/p55 (Tyr458, Tyr199)을 인식하는 재조합 토끼 단클론 항체로, PE 형광표지된 Flow Cytometry용 항체입니다. 높은 특이성과 로트 간 일관성을 제공하며, 인간 및 생쥐 시료에 반응합니다. 연구용으로만 사용 가능합니다.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PI3KY458-1A11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr458 of human phospho PI3K p85 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745034 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Phosphoinositide 3-kinase (PI3K, phospho PI3K) binds to activated (phosphorylated) protein-Tyr kinases through its SH2 domain, acting as an adapter mediating the association of the p110 catalytic unit to the plasma membrane.
Phospho PI3K is essential for insulin-stimulated glucose uptake and glycogen synthesis in insulin-sensitive tissues. It also binds to IRS-1 upon insulin stimulation. Growth factors and hormones trigger this phosphorylation event, coordinating cell growth, cell cycle entry, migration, and survival.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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