Thermo Fisher Scientific Phospho-PI3K p85/p55 (Tyr458, Tyr199) Recombinant Rabbit Monoclonal Antibody (PI3KY458-1A11)
PI3K p85/p55 단백질의 Tyr458 및 Tyr199 인산화 부위를 인식하는 재조합 토끼 단일클론 항체. 높은 특이성과 재현성을 제공하며 Flow Cytometry에 적합. Human 및 Mouse 시료에 반응. 단백질 A/G 정제, PBS/glycerol buffer에 보관.
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Applications
- Flow Cytometry (Flow): 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PI3KY458-1A11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr458 of human phospho PI3K p85. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745035 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Phosphoinositide 3-kinase (PI3K, phospho PI3K) binds to activated (phosphorylated) protein-Tyr kinases via its SH2 domain, mediating association of the p110 catalytic unit to the plasma membrane.
Phospho PI3K is essential for insulin-stimulated glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it binds to IRS-1. Growth factors and hormones trigger phosphorylation events that coordinate cell growth, cycle entry, migration, and survival.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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