Thermo Fisher Scientific ULK1 Polyclonal Antibody
ULK1 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간, 생쥐, 랫트 반응성. Western blot 및 IHC에 사용 가능. 자가포식 초기 단계 연구에 적합하며, 동결건조 형태로 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:200–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the N-terminal region of mouse ULK1 (ATG1) conjugated to an immunogenic carrier protein. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: ULK1.
Target Information
ULK1 is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy, a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response and rapamycin-induced autophagy, consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13, essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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