Thermo Fisher Scientific ULK1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:200–1:2,000

Immunohistochemistry (IHC)

• Tested Dilution: 1:200–1:2,000

Miscellaneous (PubMed)

• Publications: View 2 publications

Product Specifications

Product Specific Information

Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity: ULK1. This antibody can recognize both the phosphorylated and unphosphorylated target.

Target Information

ULK1 is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy, a vital response to nutrient starvation.
The conserved C-terminal domain (CTD) of ULK1 controls regulatory function and localization. Knockdown of ULK1 inhibits autophagic response and rapamycin-induced autophagy, consistent with its role downstream of mTOR.
ULK1 forms a complex with FIP200 and ATG13, essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and for ULK1 protein stability.
ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.