Thermo Fisher Scientific Acidic Cytokeratin Monoclonal Antibody (OTI7F10), TrueMAB
인간 세포에서 반응하는 Acidic Cytokeratin 단클론 항체로, IHC(파라핀 절편) 실험에 최적화되어 있습니다. 합성 펩타이드 면역원 기반으로 제작되었으며, 고순도 친화 크로마토그래피 정제. 연구용으로만 사용 가능하며, 암 진단 연구 및 세포 분화 분석에 유용.
- 판매단위
- pk
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Applications
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7F10 |
| Immunogen | Synthetic peptide corresponding to the conserved region of human type I (Acidic) keratins (including KRT9–KRT20) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Synthetic peptide sequence:
EKVTMQNLND RLASYLDKVR ALEEAN
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Cytokeratin pan belongs to a subfamily of intermediate filament proteins exhibiting remarkable biochemical diversity. In human epithelial tissues, at least 20 different cytokeratin polypeptides are expressed, ranging from 40–68 kDa with an isoelectric pH between 4.9–7.8.
Cytokeratin expression patterns vary by epithelial type, maturation stage, and disease state, making them valuable markers in cancer research and tumor diagnosis.
Cytokeratin antibodies assist in:
- Differential diagnosis of epithelial malignancies (via IHC)
- Cytopathology and flow cytometric assays
- Research on epithelial differentiation and cancer progression
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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