Thermo Fisher Scientific Acidic Cytokeratin Monoclonal Antibody (OTI8F5), TrueMAB
인간 시토케라틴을 인식하는 단클론 항체로, IHC(P)에서 1:150 희석으로 사용 가능. 합성 펩타이드 면역원 기반으로 높은 특이성과 재현성 제공. 동결건조 형태로 제공되며, -20°C에서 보관. 연구용으로 암 진단 연구 및 세포 분화 분석에 활용 가능.
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Applications
Tested Application
- Immunohistochemistry (Paraffin) (IHC (P)): 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8F5 |
| Immunogen | Synthetic peptide corresponding to the conserved region of human type I (Acidic) keratins (including KRT9, KRT10, KRT12, KRT13, KRT14, KRT15, KRT16, KRT17, KRT18, KRT19, KRT20) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Synthetic peptide sequence: EKVTMQNLND RLASYLDKVR ALEEAN
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Cytokeratin pan belongs to a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides.
Cytokeratins range in molecular weight between 40–68 kDa and have an isoelectric pH between 4.9–7.8.
Human cytokeratins are numbered 1 to 20, and their expression patterns are characteristic of epithelial type and degree of differentiation.
These patterns are valuable in distinguishing epithelial malignancies, aiding in tumor differential diagnosis through immunohistochemistry, cytopathology, and flow cytometry.
Cytokeratin composition varies by epithelial cell type, differentiation stage, growth environment, and disease state.
Keratins are widely used as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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