Thermo Fisher Scientific Acidic Cytokeratin Monoclonal Antibody (OTI8F5), TrueMAB

Applications

Tested Application

• Immunohistochemistry (Paraffin) (IHC (P)): 1:150

Product Specifications

Product Specific Information

Synthetic peptide sequence: EKVTMQNLND RLASYLDKVR ALEEAN

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Cytokeratin pan belongs to a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides.
Cytokeratins range in molecular weight between 40–68 kDa and have an isoelectric pH between 4.9–7.8.

Human cytokeratins are numbered 1 to 20, and their expression patterns are characteristic of epithelial type and degree of differentiation.
These patterns are valuable in distinguishing epithelial malignancies, aiding in tumor differential diagnosis through immunohistochemistry, cytopathology, and flow cytometry.

Cytokeratin composition varies by epithelial cell type, differentiation stage, growth environment, and disease state.
Keratins are widely used as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.