Thermo Fisher Scientific PKM1 Monoclonal Antibody (OTI4E8), TrueMAB
PKM1 단백질을 인식하는 mouse IgG1 단일클론 항체로, Western blot 및 IHC(P) 실험에 적합합니다. 합성 펩타이드 기반 면역원으로 제작되었으며, carrier-free 형태로 재결합 및 conjugation 실험에 활용 가능합니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4E8 |
| Immunogen | Synthetic peptide sequence corresponding to the region encoded by PKM gene M1 isoform |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Synthetic peptide sequence: LVRASSHSTD LMEAMAMGSV
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
PKM1 encodes a protein involved in glycolysis. The encoded pyruvate kinase catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate.
This protein interacts with thyroid hormone and may mediate metabolic effects induced by thyroid hormones. It also binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence and invasion of human cells, suggesting a role in bacterial pathogenesis.
Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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