Thermo Fisher Scientific PDGFRA Recombinant Superclonal Antibody
Recombinant Superclonal™ rabbit antibody targeting human PDGFRA (CD140a). Offers high sensitivity and specificity with consistent lot-to-lot performance. Suitable for WB, IHC, ICC/IF, and ChIP applications. Ideal for detecting low-abundance targets in ...
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–5 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1–3 µg/mL | - |
| ChIP assay (ChIP) | 1 µL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to amino acids 259–436 of human platelet-derived growth factor receptor alpha chain (PDGFRA, CD140a, UniProt ID: P16234-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532600 |
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They combine multiple recombinant monoclonal antibodies, offering the sensitivity of polyclonal antibodies and the specificity of monoclonal antibodies, with the consistency of recombinant production.
Each lot contains a known mixture of light and heavy chains, ensuring reproducibility and eliminating biological variability typical of polyclonal antibody production.
Note: Formerly called “Recombinant polyclonal antibody”, now rebranded as “Recombinant Superclonal™ antibody”. The product and performance remain unchanged.
Target Information
PDGFRA is a cell surface receptor tyrosine kinase binding platelet-derived growth factors, which are mitogens for mesenchymal-origin cells. The receptor can form homodimers or heterodimers with PDGFRB, depending on the bound growth factor.
Studies in knockout mice show that the alpha form is crucial for kidney development, as heterozygous mice display defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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