Thermo Fisher Scientific ECS (DYKDDDDK) Tag Polyclonal Antibody
DYKDDDDK(FALG) 태그 인식용 고트 폴리클로날 항체로, WB·ELISA·ICC·IP 등 다양한 응용 가능. KLH 결합 면역원으로 제작되어 높은 특이성과 재현성을 제공. 단백질 정제 및 검출 연구용으로 적합.
- 카탈로그번호
- A190101A
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 – 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:100 – 1:400 |
| ELISA | 1:1,000 – 1:30,000 |
| Immunoprecipitation (IP) | 1 – 4 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ECS, Enterokinase Cleavage Site (DYKDDDDK) conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Goats were immunized with ECS, Enterokinase Cleavage Site (DYKDDDDK) conjugated to KLH.
Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence commonly used as a protein tag for purification, detection, and localization.
The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys.
It is typically fused to the N- or C-terminus of a protein of interest, enabling easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is widely used in molecular biology and biochemistry research, including protein-protein interaction studies, protein expression analysis, and localization studies.
Its hydrophilic nature minimizes protein denaturation or inactivation.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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