Thermo Fisher Scientific EGFR Polyclonal Antibody
EGFR 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot, IHC, IP에 적합. 인간, 마우스, 랫트 시료에서 반응하며, 고순도 친화 크로마토그래피로 정제됨. PBS 버퍼에 0.2% 젤라틴 포함, 4°C 보관.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 | - |
| Immunoprecipitation (IP) | 1–2 µg/mL | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the carboxy terminal region of human EGFR |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 0.2% gelatin |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_325381 |
Product Specific Information
OPA1-10100 detects EGFR from human, mouse, and rat samples.
It has been successfully used in Western blot, immunohistochemistry, and immunoprecipitation procedures.
The immunizing peptide corresponds to the carboxy terminal region of human EGFR.
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that signals in response to various growth factors.
Overexpression has been linked to numerous types of cancer, and EGFR is the target of both biological and small molecule therapeutics.
EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ERbB family of proteins, which includes ERbB2, ERbB3, and ERbB4.
Binding of ligands such as EGF and TGF-α to the extracellular domain leads to EGFR dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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