Thermo Fisher Scientific TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody (SPM542)
TGF-alpha 단백질을 인식하는 고특이성 단클론 항체로, 인간·토끼·제브라피쉬 반응성. IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. Protein A/G 정제 및 PBS buffer 제공. 연구용으로만 사용.
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Thermo Fisher Scientific TGF-alpha Monoclonal Antibody (SPM542)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Rabbit, Zebrafish |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM542 |
| Immunogen | A 10-amino acid synthetic peptide (aa34–43, PPVAAAVVSH) from human TGF-alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor and stimulates proliferation of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells, and induces anchorage-independent transformation in cultured cells. Human, murine, and rat TGF-alpha are cross-species reactive. TGF-alpha (aa 50) shares 33% homology with EGF, binds to EGFR, activates receptor tyrosine phosphorylation, and promotes cell proliferation. It plays a role in tumor initiation by inducing reversible transformed phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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