Thermo Fisher Scientific COX2 Monoclonal Antibody (COX 229), PE-Cyanine7, eBioscience

Thermo Fisher Scientific COX2 Monoclonal Antibody (COX 229), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Intracellular staining)

Tested Dilution: 0.125 µg/test

Product Specifications

Additional Formats

• Alexa Fluor 488
• Alexa Fluor 647
• eFluor 450
• PE
• Request custom conjugation

Product Specific Information

This COX 229 monoclonal antibody recognizes human COX2 (PTGS2). It has been reported to cross-react with mouse and rat COX2. For optimal performance, use with the Foxp3 / Transcription Factor Staining Buffer Set and protocol.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on stimulated normal human peripheral blood cells using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended ≤0.125 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Antibody should be titrated for optimal performance.

Light Sensitivity: Tandem dye is sensitive to photo-induced oxidation; protect from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be determined empirically.

Excitation / Emission / Laser:

• Excitation: 488–561 nm
• Emission: 775 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a key step in prostanoid synthesis including inflammatory prostaglandins. The process involves:

1. Cyclooxygenase (COX) reaction: arachidonate → prostaglandin G2 (PGG2)
2. Peroxidase reaction: PGG2 → prostaglandin H2 (PGH2)

COX2 is constitutively expressed in endothelium, kidney, and brain, and up-regulated in pathological conditions such as cancer and inflammation. Overexpression is linked to increased cell adhesion, resistance to apoptosis, and tumor angiogenesis. COX2 mediates production of prostaglandin E2 (PGE2), influencing motility and proliferation. It is inhibited by calcitriol and glucocorticoids, and targeted by NSAIDs like aspirin.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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