Thermo Fisher Scientific KLRG1 Monoclonal Antibody (13F12F2), PE-Cyanine7, eBioscience

Thermo Fisher Scientific KLRG1 Monoclonal Antibody (13F12F2), PE-Cyanine7, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.06 µg)/test
Publications: View 6 publications

Product Specifications

Available Formats

• Alexa Fluor 488
• Alexa Fluor 647
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 786
• Functional Grade
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitory receptor of the C-type lectin superfamily containing an ITIM domain.
KLRG1 is expressed on subsets of γδ T cells, NK (CD56^dim), CD8+ and CD4+ T cells, primarily by effector/memory phenotype cells that are short-lived but capable of immediate effector function.
Cadherin-E, -N, and -R are ligands for KLRG1, and their interaction inhibits cytolytic activity and proliferation. The percentage of KLRG1-positive cells varies depending on antigen experience.

The clones 13F12F2 and 13A2 recognize a similar epitope based on cross-blocking studies.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (0.06 µg) per test (100 µL final volume). Cell number range: 10^5–10^8 cells/test.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation/Emission: 488–561 nm / 775 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family with immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic domains.
ITIMs interact with SH2 domains of phosphatases such as SHP-1.
KLRG1 is expressed on NK cells and certain T cells, which mediate cytolysis of tumor and virus-infected cells and participate in immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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