Thermo Fisher Scientific hnRNP K Recombinant Rabbit Monoclonal Antibody (SC60-03)
Western blot, IHC, ICC/IF에 적합한 재조합 토끼 단클론 항체로 높은 특이성과 재현성을 제공. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC60-03 |
| Immunogen | Synthetic peptide within Human hnRNP K (aa 1–49) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809497 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened for optimal performance.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
The hnRNP K gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, mRNA metabolism, and transport.
While most hnRNPs reside in the nucleus, some shuttle between the nucleus and cytoplasm. hnRNP K contains three KH RNA-binding domains and preferentially binds to poly(C) sequences. It also plays a role in cell cycle progression. Several alternatively spliced transcript variants exist, though not all are fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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