Thermo Fisher Scientific Phospho-TrkB (Tyr817) Recombinant Rabbit Monoclonal Antibody (SC0556)
Phospho-TrkB (Tyr817) 특이적 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF에 사용 가능. 높은 특이성과 민감도, 배치 간 일관성 및 동물 유래 성분 무첨가. 신경계 발달 및 시냅스 기능 연구에 적합.
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Thermo Fisher Scientific Phospho-TrkB (Tyr817) Recombinant Rabbit Monoclonal Antibody (SC0556)
Applications and Tested Dilution
- Western Blot (WB): 1:1,000–1:5,000
- Immunohistochemistry (Paraffin) [IHC (P)]: 1:1,000
- Immunocytochemistry (ICC/IF): 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC0556 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr817 of Human TrkB (aa 789–822) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809494 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
NTRK2 (TRKB) encodes a receptor for brain-derived neurotrophic factor (BDNF), playing a crucial role in development and maintenance of the mammalian nervous system.
- Regulates short-term synaptic functions and long-term potentiation.
- TrkB is a tyrosine kinase receptor expressed in central and peripheral nervous tissues.
- BDNF and NT-3 induce tyrosine phosphorylation of TrkB; BDNF response is significantly stronger.
- TrkB activation triggers MAPK pathway signaling, leading to cell differentiation.
- Mutations are linked to obesity and mood disorders.
- Multiple splice variants exist for TrkB.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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